Cells Can Be Labeled and then Frozen for Later Use
The image below shows how Molday ION Rhodamine B™ can be loaded into cells, with the cells cryogenically stored, thawed, and still have the label contained within viable cells. A) Rhodamine prior to cryogenic freezing, B) Ph2 thawed 2 weeks later, C) paired Rhodamine channel, thawed cells. Click to enlarge.
The Fluorescent Label is Covalently Bonded to the USPIO
The image below shows NIH-3T3 cells loaded with Molday ION Rhodamine B. The cells were fixed, then stained for iron using Prussian Blue Reagent. A) Ph1 B stain B) Rhodamine image. (200X magnification, bar=20µm)
TEM Images of Labeled Cells
The TEM image below to the left represents a cell loaded with Molday ION Rhodamine B, (25ugFe/ml), magnified 4600X. Dark vesicles can be seen inside the cytoplasm of the cell, each vesicle is packed with the nanoparticle. The white square outlines the area magnified, represented in the image to the right. bar = 2 µm.
The image below to the right is a 36000X magnified TEM showing two vesicles loaded with Molday ION Rhodamine B. The small dark spheres are the iron oxide core of the nanoparticles. bar = 200 nm. Click to enlarge images.