Cell Labeling, Tracking, Imaging and Magnetic Targeting...

Cell delivery and retention are impartment parameters for researchers in regenerative medicine and cellular therapy to evaluate and understand. Therefore, reliable cell labeling and tracking tools can accelerate the development process for drug developers.

Molday ION Rhodamine B™ is a commercially available product to label and track cells by MRI imaging. This product is homogeneous iron oxide based nanoparticle (USPIO) with a diameter of approximately 35nm. Thus far, all cells treated with Molday ION Rhodamine B cleanly internalize this nano-material. Molday ION Rhodamine B localizes to endosomes, does not localize to the nucleus, mitochondria, endoplasmic reticulum, Golgi apparatus, or any other cellular organelle, nor is it found on the surface of cells post labeling. Molday ION Rhodamine B labels cells efficiently, is retained within the cell for extended time periods, and is well tolerated. The labeling procedure is quick, safe, easy, and does not require the use of transfection reagents. Cell labeling requires adding Molday ION Rhodamine B directly to cells in media. Molday ION Rhodamine B offers: (i) the ability to label cells in vitro and then track the cells in vivo. Tracking is accomplished using MRI, fluorescence, or staining of biopsied samples; (ii) opportunity to conjugate ligands for specific targeting and imaging applications in vitro or in vivo; (iii) multiple platforms for dual drug delivery and imaging system; (iv) magnetic manipulation for targeting in vivo, isolation from tissues and post labeling purification; (v) magnetic/RF hyperthermic treatment.

Our stem cell labeling and MRI tracking product can be made available for human clinical trials.

Molday ION Rhodamine B™

Click to enlarge.

Stem Cell Labeling Agent.

Stem Cell Tracking Agent.

Magnetic Targeting Stem Cells.

Stem cell tracking product.

Inquire about using BioPAL's therapeutic cell labeling, tracking and magnetic retention product for use in human clinical applications.

Examples of Labeled Cells

Below are seven photomicrographs depicting different cell types labeled with Molday ION Rhodamine B™. The images are paired ph1 contrast and fluorescence. Click to enlarge images.

Adherent Cells:

Stem cell labeling and tracking by MRI and magnetic targeting. Stem cell labeling and tracking by MRI and magnetic targeting. Stem cell labeling and tracking by MRI and magnetic targeting.

Stem Cells:

Stem cell labeling and tracking by MRI and magnetic targeting. Stem cell labeling and tracking by MRI and magnetic targeting.

Primary Cells:

Stem cell labeling and tracking by MRI and magnetic targeting.

Suspension Cells:

Stem cell labeling and tracking by MRI and magnetic targeting.

Imaging probes and nanoprobes for magnetic resonance imaging (MIR) imaging of stem cell tracking and magnetic targeting and retention.

Stem cell tracking product. Magnetic targeting of stem cells.

USPIO specifically designed to label therapeutic cells and stem cell and the track them using MRI and magnetic targeting and retention.

Cells Can Be Labeled and then Frozen for Later Use

The image below shows how Molday ION Rhodamine B™ can be loaded into cells, with the cells cryogenically stored, thawed, and still have the label contained within viable cells. A) Rhodamine prior to cryogenic freezing, B) Ph2 thawed 2 weeks later, C) paired Rhodamine channel, thawed cells. Click to enlarge.

Cell labeling and tracking product. Stem cell retention product.

The Fluorescent Label is Covalently Bonded to the USPIO

The image below shows NIH-3T3 cells loaded with Molday ION Rhodamine B. The cells were fixed, then stained for iron using Prussian Blue Reagent. A) Ph1 B stain B) Rhodamine image. (200X magnification, bar=20µm)

USPIO cell labeling product. USPIO stem cell retention product.

TEM Images of Labeled Cells

The TEM image below to the left represents a cell loaded with Molday ION Rhodamine B, (25ugFe/ml), magnified 4600X. Dark vesicles can be seen inside the cytoplasm of the cell, each vesicle is packed with the nanoparticle. The white square outlines the area magnified, represented in the image to the right. bar = 2 µm.

The image below to the right is a 36000X magnified TEM showing two vesicles loaded with Molday ION Rhodamine B. The small dark spheres are the iron oxide core of the nanoparticles. bar = 200 nm. Click to enlarge images.

Stem cell research product to label and track therapeutic cells by MRI for preclinical and human clinical applications.

Stem cell research product for imaging therapeutic cells for magnetic retention of cells in preclinical and human clinical applications.

The Product

Molday ION Rhodamine B™ (MIRB) is BioPAL’s most popular cell labeling and tracking product. It is a 35 nm rhodamine B-labeled iron-based superparamagnetic contrast agent (USPIO) packaged in a 2 ml sealed serum bottle. However, BioPAL also offers the product without the Rhodamine dye or with alternative fluorescent dyes, such as a dye for Near Infrared Imaging (NIR).

These products are all 35 nm iron-based superparamagnetic contrast agents (USPIO) packaged in a 2 ml sealed serum bottle. The bottle fill and concentration will vary for each product, but the specific details for each product are provided on the data sheet provided below. Both CL-50Q02-6A-0 and CL-50Q02-6A-50 are also offered autoclaved.
Research Use ONLY. Not For Human Use.

Researchers may order BioPAL’s cell labeling and tracking probes online using the add-to-cart button below. Alternatively, researchers can phone, fax or e-mail a purchasing request to BioPAL. These instructions can be found on the main navigation bar above. For more information about our company, please visit our main web site at BioPAL.com.


Cat. No. Product Name Price (US) Data Sheet MSDS  
CL-50Q02-6A-0 Molday ION™ Dye Free $375.00 MRI cell labeling, tracking and imaging agent Stem cell labeling and tracking.
CL-50Q01-6A-50 Molday ION Rhodamine B™ (MIRB) $140.00 MRI cell labeling, tracking and imaging agent Stem cell labeling and tracking.
CL-50Q02-6A-50 Molday ION Rhodamine B™ (MIRB) $450.00 MRI cell labeling, tracking and imaging agent Stem cell labeling and tracking.
CL-50Q02-6A-51 Molday ION™ EverGreen $450.00 MRI cell labeling, tracking and imaging agent Stem cell labeling and tracking.
CL-50Q02-6A-52 Molday ION™ Coumarin $450.00 MRI cell labeling, tracking and imaging agent Stem cell labeling and tracking.
CL-50Q01-6A-53 Molday ION™ MI-750 $400.00 MRI cell labeling, tracking and imaging agent Stem cell labeling and tracking.
CL-50Q02-A6A-0 Autoclaved Molday ION™ Dye Free $450.00 MRI cell labeling, tracking and imaging agent Stem cell labeling and tracking.
CL-50Q02-A6A-50 Autoclaved MIRB $475.00 MRI cell labeling, tracking and imaging agent Stem cell labeling and tracking.

Cell labeling product to label cells and then track cells and image cells using MRI and florescence imaging. Primary cells, T-cells, stems cells and platelets can be labeled, imaged and tracked.

BioPAL, Inc. • 80 Webster Street Worcester, MA 01603 • TEL: 508-770-1190 • FAX: 508-770-1191 • e-mail: cs@BioPAL.com

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